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Related Experiment Video

Updated: Jan 19, 2026

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Published on: January 20, 2017

A robust human norovirus replication model in zebrafish larvae.

Jana Van Dycke¹, Annelii Ny², Nádia Conceição-Neto³

¹KU Leuven-Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Virology and Chemotherapy, Leuven, Belgium.

|September 20, 2019

Summary

This summary is machine-generated.

Human noroviruses (HuNoVs) cause significant foodborne illness. Zebrafish larvae now offer a robust model for studying HuNoV replication and developing new antiviral treatments.

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Last Updated: Jan 19, 2026

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Published on: November 27, 2014

Area of Science:

Virology
Infectious Diseases
Animal Models

Background:

Human noroviruses (HuNoVs) are a leading cause of foodborne illness globally.
The absence of effective small animal models impedes norovirus research and therapeutic development.

Purpose of the Study:

To establish and validate a novel small animal model for human norovirus replication.
To assess the utility of this model for antiviral drug screening.

Main Methods:

Infection of zebrafish (Danio rerio) larvae with human norovirus (HuNoV) GI and GII strains.
Monitoring viral replication titers over time (up to 6 days post-infection).
Serial passaging of the virus between larvae and assessment of viral tropism in host tissues.

Main Results:

High-titer replication of HuNoV GI and GII was observed in zebrafish larvae, peaking at day 2 post-infection.
The GII.4 strain demonstrated successful serial passaging between larvae, indicating sustained infectivity.
HuNoV was detected in hematopoietic and intestinal cells, suggesting dual tissue tropism.
Antiviral treatment significantly reduced viral replication (>2 log10), confirming model suitability for drug studies.

Conclusions:

Zebrafish larvae provide a simple, robust, and effective model for human norovirus replication.
This model system will accelerate the understanding of norovirus pathogenesis.
The validated model is well-suited for high-throughput screening of antiviral compounds against noroviruses.