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Updated: Jan 19, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
[The mass-spectrometry as a new word in clinical microbiology]
V M Priputnevich1, A R Melkumyan1
1The academician V.I. Kulakov research center of obstetrics, gynecology and perinatology of Minzdrav of Russia, 117997, Moscow, Russia.
Abstract:
In the end of XX century real revolution occurred in field of methods, which can be applied for identification of agents of infections including bacteria. Alongside with cultural diagnostic entered an arsenal of molecular genetic methods and proteomic analysis based on application of physical technologies including matrix activated laser desorption/ionization time-of-flight mass spectrometry - MALDI-ToF MS as well. The international experience of application of MALDI-ToF MS as species identification of microorganisms isolated from clinical samples substantiates high importance of method. The potential possibility of implementing direct indication of bacteria in clinical samples significantly shorten period of implementation of analysis and reveals new resources for implementing in various algorithms of microbiological diagnostic.
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