Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.8K
MicroRNAs01:22

MicroRNAs

24.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.0K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

18.5K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
18.5K
Types of RNA01:23

Types of RNA

72.6K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
72.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring per-base quality scores as a surrogate marker of cell-free DNA fragmentome.

Briefings in bioinformatics·2026
Same author

Opposite molecular sex correlations in tauopathy paralleled by motor and cognitive efficacy of davunetide in women.

Molecular psychiatry·2026
Same author

FOXM1 expression is induced by the brain microenvironment and supports CRC brain metastatic adaptation.

Clinical & experimental metastasis·2026
Same author

Gut microbiota composition correlates with PBMC microRNA expression following maximal exercise testing in endurance athletes.

Frontiers in microbiomes·2026
Same author

Artificial Intelligence Does Not Always Win.

The Israel Medical Association journal : IMAJ·2026
Same author

Revisiting low penetrance retinoblastoma: an integrated clinical, genetic, and bioinformatic analysis.

Human molecular genetics·2026

Related Experiment Video

Updated: Jan 19, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

7.9K

MicroRNAs Affect Complement Regulator Expression and Mitochondrial Activity to Modulate Cell Resistance to

Yaron Hillman1, Mariya Mardamshina2, Metsada Pasmanik-Chor3

  • 1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.

Cancer Immunology Research
|September 21, 2019
PubMed
Summary

MicroRNAs regulate resistance to complement-dependent cytotoxicity (CDC). Specific microRNAs (miR-150, miR-328, miR-616) impact cell survival by modulating complement regulators and mitochondrial response to CDC.

More Related Videos

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.8K
A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
06:30

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Published on: January 27, 2021

2.1K

Related Experiment Videos

Last Updated: Jan 19, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

7.9K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.8K
A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
06:30

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Published on: January 27, 2021

2.1K

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are crucial regulators of gene expression, influencing cellular processes.
  • Complement-dependent cytotoxicity (CDC) is a key immune mechanism targeting cells.
  • Understanding miRNA roles in CDC resistance is vital for therapeutic development.

Purpose of the Study:

  • To investigate the role of microRNAs in determining cellular resistance to complement-dependent cytotoxicity (CDC).
  • To identify specific microRNAs that regulate cell sensitivity or resistance to CDC.

Main Methods:

  • Comparative microRNA expression analysis between CDC-resistant and -sensitive cancer cell lines (K562, Raji, HCT-116).
  • Global microRNA array profiling to identify key miRNA regulators.
  • Inhibition of specific miRNAs to assess effects on CDC resistance.
  • Mass spectrometry-based proteomics to identify protein targets of regulatory miRNAs.

Main Results:

  • miR-150, miR-328, and miR-616 were identified as key regulators of CDC resistance.
  • Inhibition of miR-150 decreased resistance, while inhibiting miR-328 or miR-616 increased resistance.
  • Complement treatment rapidly upregulated miR-150, miR-328, and miR-616.
  • miRNA inhibition affected expression of complement regulators CD46 and CD59.
  • Mitochondrial protein enrichment was observed upon inhibition of these miRNAs, indicating altered response to complement attack.

Conclusions:

  • miR-150, miR-328, and miR-616 are critical modulators of cellular resistance to CDC.
  • These miRNAs influence CDC resistance by altering CD46 and CD59 expression and mitochondrial response.
  • Targeting these microRNAs offers potential therapeutic strategies for complement-associated diseases and anti-cancer therapies.