Complete Genome Sequence of Escherichia coli Myophage Mansfield

Genevieve M D'Souza1,2, Kathryn Klotz1, Russell Moreland1

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Insights

Mansfield, an Escherichia bacteriophage, possesses a large genome encoding 105 proteins. Researchers predicted the functions of 32 of these proteins, contributing to phage biology understanding.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Bacteriophages are viruses that infect bacteria, playing crucial roles in microbial ecosystems.
  • Understanding phage genomes is essential for their application in biotechnology and medicine.

Purpose of the Study:

  • To characterize the genome of the Escherichia bacteriophage Mansfield.
  • To identify and predict the functions of proteins encoded by the Mansfield phage.

Main Methods:

  • Genome sequencing and analysis of the Escherichia bacteriophage Mansfield.
  • Bioinformatic prediction of protein functions.

Main Results:

  • The Mansfield phage has a genome size of 68,120 base pairs.
  • A direct terminal repeat of 3,673 base pairs was identified.
  • The genome encodes 105 proteins, with functions predicted for 32 of them.

Conclusions:

  • Mansfield is a PB1-like bacteriophage with a well-defined genome structure.
  • The predicted protein functions provide insights into the biology of this Escherichia phage.

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