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Complete Genome Sequence of Escherichia coli Myophage Mansfield
Genevieve M D'Souza1,2, Kathryn Klotz1, Russell Moreland1
1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.
Abstract:
Mansfield is a PB1-like Escherichia bacteriophage with a 68,120-bp genome and a predicted 3,673-bp direct terminal repeat. This myophage encodes 105 proteins, for which 32 functions were predicted.
Insights
Mansfield, an Escherichia bacteriophage, possesses a large genome encoding 105 proteins. Researchers predicted the functions of 32 of these proteins, contributing to phage biology understanding.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Bacteriophages are viruses that infect bacteria, playing crucial roles in microbial ecosystems.
- Understanding phage genomes is essential for their application in biotechnology and medicine.
Purpose of the Study:
- To characterize the genome of the Escherichia bacteriophage Mansfield.
- To identify and predict the functions of proteins encoded by the Mansfield phage.
Main Methods:
- Genome sequencing and analysis of the Escherichia bacteriophage Mansfield.
- Bioinformatic prediction of protein functions.
Main Results:
- The Mansfield phage has a genome size of 68,120 base pairs.
- A direct terminal repeat of 3,673 base pairs was identified.
- The genome encodes 105 proteins, with functions predicted for 32 of them.
Conclusions:
- Mansfield is a PB1-like bacteriophage with a well-defined genome structure.
- The predicted protein functions provide insights into the biology of this Escherichia phage.
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