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Updated: Jan 19, 2026

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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MicroRNA-199a-5p regulates glioma progression via targeting MARCH8

C Zhang1, Q Chen, J-W Zhu

  • 1Department of Neurosurgery Ward 1, Hanzhong Central Hospital, Hanzhong, P.R. China. liuzhenfeng_xy@126.com.

European Review for Medical and Pharmacological Sciences
|September 21, 2019
PubMed
Abstract

Insights

MicroRNA-199a-5p is downregulated in glioma. Overexpressing this microRNA suppresses glioma cell growth and invasion by targeting MARCH8, suggesting its tumor suppressor role.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNA-199a-5p (miR-199a-5p) is implicated in cancer progression.
  • The specific function of miR-199a-5p in glioma is not well understood.

Purpose of the Study:

  • To investigate the role of miR-199a-5p in glioma.
  • To identify the molecular targets of miR-199a-5p in glioma cells.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) to assess miR-199a-5p expression.
  • In vitro assays to evaluate the impact of miR-199a-5p on glioma cell proliferation and invasion.
  • Bioinformatic analysis and Luciferase assay to identify miR-199a-5p targets.

Main Results:

  • miR-199a-5p expression was significantly reduced in glioma cell lines compared to normal cells.
  • Overexpression of miR-199a-5p inhibited glioma cell proliferation and invasion.
  • The membrane-associated ring-CH-type finger 8 (MARCH8) was identified as a direct target of miR-199a-5p.
  • MARCH8 re-expression partially reversed the tumor-suppressive effects of miR-199a-5p.

Conclusions:

  • miR-199a-5p acts as a tumor suppressor in glioma.
  • The miR-199a-5p/MARCH8 axis is a potential therapeutic target for glioma treatment.

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