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Related Experiment Video

Updated: Jan 19, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Multi-focus microscope with HiLo algorithm for fast 3-D fluorescent imaging.

Wei Lin^1,2, Dongping Wang¹, Yunlong Meng¹

¹Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong.

|September 21, 2019

Summary

This summary is machine-generated.

This study introduces a novel multi-focus microscope (MFM) for high-speed, high-resolution 3-D fluorescent imaging. The system effectively reduces noise and enhances image quality for biological samples.

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Area of Science:

Biophotonics
Microscopy
Optical Imaging

Background:

3-D fluorescent imaging is crucial for biological research.
Existing methods often face limitations in speed, resolution, or noise levels.

Purpose of the Study:

To develop a high-speed, high-resolution, low-noise 3-D fluorescent imaging system.
To demonstrate the system's capability in visualizing biological samples.

Main Methods:

A multi-focus microscope (MFM) system utilizing a phase mask and HiLo algorithm.
Simultaneous modulation and focusing of emissions from nine different depths onto a single CCD chip.
Axial scanning for larger volume visualization.

Main Results:

Achieved high-speed imaging at 20 volumes per second.
Demonstrated high-resolution, low-noise 3-D fluorescent imaging.
Successfully visualized a mouse kidney sample (~60 × 60 × 16 μm3) with enhanced resolution, contrast, and suppressed background noise using only 10 raw images.

Conclusions:

The developed MFM system offers significant advancements in 3-D fluorescent imaging.
The method enables fast dynamic event recording at multiple depths in vivo.
Potential for broad applications in biological and medical imaging.