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Updated: Jan 19, 2026
Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
Proliferative Effect of Tilapia Fish (Oreochromis niloticus) Lectin on BALB/c Mice Splenocytes
Cynarha Daysy Cardoso da Silva1, Cristiane Moutinho Lagos de Melo2, Elba Verônica Matoso Maciel Carvalho1
1Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Background:
Lectins have been studied in recent years due to their immunomodulatory activities.
Objective:
We purified a lectin named OniL from tilapia fish (Oreochromis niloticus) and here we analyzed the cell proliferation and cytokine production in Balb/c mice splenocytes.
Methods:
Cells were stimulated in vitro in 24, 48, 72 hours and 6 days with different concentrations of OniL and Con A. Evaluation of cell proliferation was performed through [3H]-thymidine incorporation, cytokines were investigated using ELISA assay and cell viability assay was performed by investigation of damage through signals of apoptosis and necrosis.
Results:
OniL did not promote significant cell death, induced high mitogenic activity in relation to control and Con A and stimulated the cells to release high IL-2 and IL-6 cytokines.
Conclusion:
These findings suggest that, like Con A, OniL lectin can be used as a mitogenic agent in immunostimulatory assays.
