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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

2.8K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Types Of Column Chromatography01:29

Types Of Column Chromatography

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Updated: Jan 19, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
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Affinity Chromatography: An Enabling Technology for Large-Scale Bioprocessing.

Karol M Łącki1, Frank J Riske2

  • 1Avitide Inc., Lebanon, NH, 03766, USA.

Biotechnology Journal
|September 24, 2019
PubMed
Summary
This summary is machine-generated.

Affinity chromatography (AC) is a key method in biopharmaceutical manufacturing, offering economic benefits through process standardization and continuous operations. Custom resins enhance AC for both capture and polishing, improving drug substance quality.

Keywords:
affinitybioprocesschromatographycontinuous operationcustom resinsfacility platform

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Area of Science:

  • Biopharmaceutical Manufacturing
  • Chromatographic Separation Science
  • Process Engineering

Background:

  • Affinity chromatography (AC) has been a cornerstone of biopharmaceutical downstream processing for nearly four decades.
  • It is the preferred method for primary capture of diverse biopharmaceuticals.
  • The evolution of AC includes platform processes and custom-developed affinity resins.

Purpose of the Study:

  • To review the historical development of bioprocess AC.
  • To examine current platform processes and custom affinity resins.
  • To discuss the advantages of AC and its future in bioprocessing.

Main Methods:

  • Literature review and synthesis of existing research on affinity chromatography in bioprocessing.
  • Analysis of economic and operational advantages of AC.
  • Exploration of custom affinity resins for capture and polishing applications.

Main Results:

  • AC offers significant economic advantages through process standardization and continuous manufacturing.
  • Custom affinity resins provide solutions for non-platformable processes and polishing steps.
  • AC enables precise control over drug substance composition by separating product variants.

Conclusions:

  • Affinity chromatography remains a vital technique in large-scale bioprocessing.
  • Custom affinity resins represent a maturing field with expanding applications.
  • AC facilitates efficient, standardized, and flexible biomanufacturing, improving product quality.