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Upregulated circ_0005576 facilitates cervical cancer progression via the miR-153/KIF20A axis

Hanyu Ma1, Tian Tian1, Xubin Liu1

  • 1Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|September 24, 2019
PubMed

Insights

Circular RNAs (circRNAs) promote cervical cancer (CC) progression. The novel circ_0005576 acts as a sponge for miR-153-3p, increasing KIF20A expression and driving CC cell growth and metastasis.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are emerging as critical regulators in oncogenesis.
  • The specific roles of novel circRNAs in cervical cancer (CC) progression remain largely unexplored.
  • Understanding these roles is crucial for identifying new therapeutic targets in CC.

Purpose of the Study:

  • To identify novel circRNAs involved in cervical cancer (CC) progression.
  • To elucidate the biological function and mechanism of circ_0005576 in CC.
  • To investigate the potential of the circ_0005576/miR-153-3p/KIF20A axis as a therapeutic target.

Main Methods:

  • Analysis of two Gene Expression Omnibus (GSE) microarrays to screen for CC-specific circRNAs.
  • Quantitative real-time PCR (qRT-PCR) to assess circ_0005576 expression in CC tissues and cell lines.
  • Cellular assays including knockdown, colony formation, migration, and invasion assays to evaluate circ_0005576 function.
  • Mechanism investigation involving RNA immunoprecipitation (RIP) and dual-luciferase reporter assays to confirm the interaction between circ_0005576, miR-153-3p, and KIF20A.

Main Results:

  • Two novel circRNAs were identified in CC, with circ_0005576 significantly overexpressed in CC tissues and cell lines.
  • Elevated circ_0005576 levels correlated with advanced FIGO stage, lymph node metastasis, and poorer overall survival in CC patients.
  • Knockdown of circ_0005576 suppressed CC cell proliferation, colony formation, and metastasis.
  • Mechanistically, circ_0005576 acts as a molecular sponge for miR-153-3p, leading to increased expression of kinesin family member 20A (KIF20A).
  • Rescue experiments confirmed the role of the circ_0005576/miR-153-3p/KIF20A axis in promoting CC progression.

Conclusions:

  • A novel circ_0005576/miR-153-3p/KIF20A axis significantly promotes cervical cancer progression.
  • Circ_0005576 may serve as a potential biomarker for CC diagnosis and prognosis.
  • Targeting this axis offers a promising new therapeutic strategy for cervical cancer.
Keywords:
Cervical cancer (CC)Circular RNAKIF20AmiR-153-3p

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