Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.3K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.3K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.6K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oxidovanadium(v) coordination compounds based on 1,5-bis(2-hydroxy-3-methoxybenzylidene)carbohydrazide: from discrete to polymeric assemblies.

RSC advances·2026
Same author

Advancing chloramphenicol detection through a novel porous organic polymer-enhanced screen-printed electrode.

Talanta·2026
Same author

Toll-like Receptor 7/8 Agonists Exert Antitumor Effect in a Mouse Melanoma Model.

Medicina (Kaunas, Lithuania)·2026
Same author

{Zn<sup>II</sup><sub>2</sub>} and {Zn<sup>II</sup>Au<sup>I</sup>} Metal Complexes with Schiff Base Ligands as Potential Antitumor Agents Against Human Glioblastoma Multiforme Cells.

Molecules (Basel, Switzerland)·2026
Same author

Acylation of starch in the presence of carboxylic acids and amides as organocatalysts and as hydrogen bond donors in deep eutectic solvents.

International journal of biological macromolecules·2025
Same author

Synthesis of Benzimidazol-2-ones by Oxidative Cyclization Ring Closure of Amido-Urea Precursors.

The Journal of organic chemistry·2025

Related Experiment Video

Updated: Jan 19, 2026

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
11:21

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Published on: June 26, 2010

12.5K

A novel adaptive fluorescent probe for cell labelling.

Anca G Coman1, Anca Paun1, Codruta C Popescu1

  • 1University of Bucharest, Faculty of Chemistry, Department of Organic Chemistry, Biochemistry and Catalysis, Research Centre of Applied Organic Chemistry, 90-92 Panduri Street, RO-050663 Bucharest, Romania.

Bioorganic Chemistry
|September 24, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel fluorescent compound for selectively labeling the endoplasmic reticulum (ER) in living cells. This hydrazone-based molecule exhibits low toxicity and blue emission, ideal for fluorescence microscopy applications.

Keywords:
Cell labellingEndoplasmic reticulumFluorescenceN-acylhydrazoneSynthesis

More Related Videos

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
07:38

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Published on: May 17, 2010

23.6K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.6K

Related Experiment Videos

Last Updated: Jan 19, 2026

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
11:21

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Published on: June 26, 2010

12.5K
Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
07:38

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Published on: May 17, 2010

23.6K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.6K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Organic Chemistry

Background:

  • The endoplasmic reticulum (ER) is a critical organelle involved in protein and lipid synthesis.
  • Selective labeling of the ER in living cells is essential for studying its dynamic functions.
  • Existing fluorescent probes may have limitations in specificity, toxicity, or spectral properties.

Purpose of the Study:

  • To synthesize and characterize a new hydrazone-based fluorescent compound.
  • To evaluate the compound's ability to selectively label the endoplasmic reticulum (ER) in living cells.
  • To investigate the compound's fluorescence properties and cell compatibility.

Main Methods:

  • Synthesis of a novel hydrazone-based fluorescent compound.
  • Characterization using Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Confocal fluorescence microscopy to assess ER labeling in yeast and mammalian cells.
  • Evaluation of fluorescence properties (pH and solvent dependence) and cell toxicity.

Main Results:

  • Successful synthesis of a new hydrazone-based fluorescent compound.
  • Selective and bright labeling of the endoplasmic reticulum (ER) in both yeast and mammalian cells.
  • Fluorescence properties were found to be dependent on DMSO/water ratio and pH.
  • NMR studies elucidated the compound's conformation in different environments.
  • The compound demonstrated low cytotoxicity and blue emission compatible with standard microscopy filters.

Conclusions:

  • A novel, hydrazone-based fluorescent probe has been developed for selective ER labeling.
  • The probe exhibits favorable photophysical properties and low cell toxicity.
  • This compound offers a valuable tool for live-cell imaging of the endoplasmic reticulum in biological research.