One pot facile green synthesis of crystalline bio-ZrO₂ nanoparticles using Acinetobacter sp. KCSI1 under room...

In this study, crystalline ZrO₂ nanoparticles were synthesized in one pot at room temperature using an extremophilic Acinetobacter sp. KCSI1 and characterized using various techniques to study its structural, optical and crystalline properties. The average size of the ZrO₂ nanoparticles was found to be 44 ± 7 nm. The XRD and Raman spectra showed the crystalline structure of ZrO_2. HRTEM and SAED images showed well aligned ordered crystal lattice nanoparticles. The zeta potential of Bio-NP of ZrO₂ has been found to be 36.5 ± 5.46 mV. The mechanical behaviour such as hardness and Young's modulus of Bio-ZrO₂ NPs were determined using atomic force microscopy (AFM) and found to be 9.206 ± 2.22 and 0.285 ± 0.13 GPa, respectively. No significant cytotoxicity for ZrO₂ nanoparticles was observed when tested with mouse fibroblast cells (L929), suggesting that the synthesized ZrO₂ nanoparticle is biocompatible and safe for environmental applications.