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Related Concept Videos

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Updated: Jan 19, 2026

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
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MORF9 Functions in Plastid RNA Editing with Tissue Specificity.

Faan Tian1, Jinfa Yu2, Ya Zhang3

  • 1Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, 350002 Fuzhou, China. 1160516017@fafu.edu.cn.

International Journal of Molecular Sciences
|September 25, 2019
PubMed
Summary

MORF9 protein is crucial for plant RNA editing in chloroplasts, but its impact varies by tissue. Loss of MORF9 significantly reduces editing in leaves and flowers, with minimal effects in roots.

Keywords:
Arabidopsis thalianaMORF9RNA editingorganellestissue-specific

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Area of Science:

  • Plant molecular biology
  • Organelle gene expression
  • RNA editing mechanisms

Background:

  • Plant organelle RNA editing converts cytidine (C) to uridine (U) at specific sites.
  • MORF family proteins, including MORF9, are essential for plant editosomes.
  • MORF9 is considered a core component involved in most chloroplast RNA editing.

Purpose of the Study:

  • To analyze the phenotypic effects of MORF9 loss-of-function in Arabidopsis.
  • To compare plastid RNA editing efficiencies in different tissues of MORF9 mutants.
  • To investigate the tissue-specific role of MORF9 in RNA editing.

Main Methods:

  • Phenotypic analysis of a T-DNA insertion MORF9 loss-of-function mutant and complementation line.
  • Comparison of plastid RNA editing efficiencies using bulk-cDNA sequencing.
  • Analysis of MORF9 mRNA and protein levels during plant senescence.

Main Results:

  • MORF9 loss significantly reduced RNA editing in rosette leaves and flowers, with some site-specific variations.
  • Roots showed a much lower impact of MORF9 loss on overall plastid RNA editing efficiency.
  • MORF9 mRNA levels decreased in senescent leaves, but protein levels remained stable.

Conclusions:

  • MORF9-mediated RNA editing is tissue-dependent in Arabidopsis.
  • The tissue-specific editing functions of MORF9 are critical for maintaining specific organelle proteomes and tissue functions.
  • MORF9's role in RNA editing is regulated differently at mRNA and protein levels during plant aging.