Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.6K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.6K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

4.0K
4.0K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.4K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.4K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

3.0K
3.0K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.2K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.2K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

2.5K
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploration of the aminomethylene linkage in carbazole-based donor-pi-acceptor molecules for AIE and hydrazine sensing.

Physical chemistry chemical physics : PCCP·2026
Same author

Localized NF-κB Inhibition Reduces Lipid Nanoparticle-Associated Inflammation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Mammalian H4K16ac regulates the spatiotemporal order of genome replication rather than gene expression.

Nucleic acids research·2025
Same author

CGGBP1 from higher amniotes restricts cytosine methylation and drives a GC-bias in transcription factor-binding sites at repressed promoters.

Transcription·2025
Same author

Silver Nanoparticles Synthesized from <i>Enicostemma littorale</i> Exhibit Gut Tight Junction Restoration and Hepatoprotective Activity via Regulation of the Inflammatory Pathway.

Pharmaceutics·2025
Same author

Cancer cell type-specific derepression of transposable elements by inhibition of chromatin modifier enzymes.

Communications biology·2025

Related Experiment Video

Updated: Jan 19, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.7K

CGGBP1 regulates CTCF occupancy at repeats.

Divyesh Patel1, Manthan Patel1, Subhamoy Datta1

  • 1HoMeCell Lab, Biological Engineering, Indian Institute of Technology Gandhinagar, Gandhinagar, Gujarat, 382355, India.

Epigenetics & Chromatin
|September 25, 2019
PubMed
Summary

Chromatin regulator CGGBP1 (CTCF-binding protein) influences where CTCF binds, particularly at repetitive DNA sequences. This interaction affects histone modifications, impacting gene regulation and genomic stability.

More Related Videos

In-Nucleus Hi-C in Drosophila Cells
11:58

In-Nucleus Hi-C in Drosophila Cells

Published on: September 15, 2021

4.7K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.9K

Related Experiment Videos

Last Updated: Jan 19, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.7K
In-Nucleus Hi-C in Drosophila Cells
11:58

In-Nucleus Hi-C in Drosophila Cells

Published on: September 15, 2021

4.7K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.9K

Area of Science:

  • * Molecular Biology
  • * Epigenetics
  • * Genomics

Background:

  • * Chromatin protein CGGBP1 (Chromatin Guanine Binding Protein 1) regulates gene expression, DNA methylation, and genomic integrity.
  • * CGGBP1 interacts with histone modifiers and CTCF complexes involved in enhancer-promoter looping.
  • * The interplay between CGGBP1 and CTCF in chromatin regulation was previously uncharacterized.

Purpose of the Study:

  • * To investigate the functional relationship between CGGBP1 and CTCF binding at repetitive DNA elements.
  • * To determine how CGGBP1 influences CTCF occupancy patterns across the genome.
  • * To elucidate the impact of CGGBP1-dependent CTCF binding on chromatin modifications.

Main Methods:

  • * Chromatin Immunoprecipitation sequencing (ChIP-sequencing) for CTCF.
  • * ChIP-sequencing for histone modifications (H3K4me3, H3K9me3, H3K27me3).
  • * Analysis of CTCF occupancy at repetitive DNA and canonical CTCF motifs.

Main Results:

  • * CGGBP1 levels dictate CTCF occupancy, with a preference for repetitive DNA over canonical CTCF motifs.
  • * CGGBP1-dependent CTCF binding sites within repeats are associated with specific histone modification patterns in flanking regions.
  • * CGGBP1 significantly alters the distribution of CTCF binding sites genome-wide.

Conclusions:

  • * CGGBP1 acts as a key regulator of CTCF binding site distribution, especially within interspersed repeats.
  • * The CGGBP1-CTCF interaction plays a crucial role in shaping chromatin structure and epigenetic landscapes at repetitive elements.
  • * This study reveals a novel mechanism of chromatin regulation involving CGGBP1 and CTCF crosstalk.