Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

57.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.5K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.7K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.7K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

630
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
630
Homologous Recombination02:31

Homologous Recombination

62.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Multimodal Framework for Organ- and Cell-Resolved Biological Aging and Longevity Intervention Discovery.

medRxiv : the preprint server for health sciences·2026
Same author

Sensing and perturbing mammalian cell states with reprogrammable ADAR sensors (RADARS).

Nature protocols·2026
Same author

Reprogramming site-specific retrotransposon activity to new DNA sites.

Nature·2025
Same author

Fanzors, a family of eukaryotic RNA-guided DNA endonucleases.

FEBS letters·2025
Same author

Precise kilobase-scale genomic insertions in mammalian cells using PASTE.

Nature protocols·2024
Same author

Rapid in silico directed evolution by a protein language model with EVOLVEpro.

Science (New York, N.Y.)·2024
Same journal

CODAvision: best practices and a user-friendly interface for rapid, customizable segmentation of medical images.

Nature protocols·2026
Same journal

A scalable high-throughput serolomics platform for profiling serum antibody responses in large-scale population-based cohorts.

Nature protocols·2026
Same journal

iMUT-seq mapping of DSB-induced mutations with high sensitivity at single-nucleotide resolution.

Nature protocols·2026
Same journal

An assay to quantify sexual commitment and stage conversion in the human malaria parasite Plasmodium falciparum.

Nature protocols·2026
Same journal

Author Correction: Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.

Nature protocols·2026
Same journal

High-throughput measurements of protein domain functions using magnetic separation.

Nature protocols·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

3.1K

SHERLOCK: nucleic acid detection with CRISPR nucleases.

Max J Kellner1, Jeremy G Koob1, Jonathan S Gootenberg2,3,4

  • 1Broad Institute of MIT and Harvard, Cambridge, MA, USA.

Nature Protocols
|September 25, 2019
PubMed
Summary
This summary is machine-generated.

We developed a CRISPR-based diagnostic platform (SHERLOCK) for rapid, ultra-sensitive DNA and RNA detection. This method combines pre-amplification with CRISPR-Cas enzymes for quick, portable results in under an hour.

More Related Videos

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

2.1K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

1.2K

Related Experiment Videos

Last Updated: Jan 19, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

3.1K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

2.1K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

1.2K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Diagnostics

Background:

  • Nucleic acid detection is crucial for clinical diagnostics and biotechnology.
  • Existing methods can be time-consuming or require complex equipment.
  • CRISPR-Cas systems offer high specificity for molecular recognition.

Purpose of the Study:

  • To provide detailed instructions for setting up the SHERLOCK diagnostic platform.
  • To enable rapid, sensitive, and portable detection of DNA and RNA.
  • To offer guidelines for assay design and optimization.

Main Methods:

  • Utilized recombinase-mediated polymerase pre-amplification for DNA/RNA.
  • Employed CRISPR-Cas13 or Cas12 enzymes for sequence-specific detection.
  • Integrated fluorescence and colorimetric readouts for results.
  • Developed guidelines for designing CRISPR RNA (crRNA) and primers.

Main Results:

  • Achieved results in under 1 hour with minimal setup time (<15 min).
  • Demonstrated multiplexed, portable, and ultra-sensitive detection capabilities.
  • Provided a robust protocol for SHERLOCK assay implementation.

Conclusions:

  • The SHERLOCK platform offers a rapid and sensitive solution for nucleic acid detection.
  • This CRISPR-based approach is suitable for clinical diagnostics and biotechnology.
  • The provided guidelines facilitate the design and application of SHERLOCK assays.