Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-Ion Imaging Native Mass Spectrometry: Unraveling the Structural Features and Dissociation Energetics of Macromolecular Assemblies.

Journal of the American Society for Mass Spectrometry·2026
Same author

Spatiotemporal Mapping of Drug Incorporation in Human Nails by MALDI-FTICR-MSI.

Journal of the American Society for Mass Spectrometry·2026
Same author

Orthogonal Conjugation via Endoglycosidase and Microbial Transglutaminase Enables Efficient Synthesis of Dual-Payload Antibody-Drug Conjugates.

ACS chemical biology·2026
Same author

Small Molecule Activators of Protein Phosphatase 2A Exert Global Stabilizing Effects on the Scaffold PR65.

JACS Au·2026
Same author

Isotope Decluttering Reduces Spectral Complexity while Maintaining Protein Structure.

Analytical chemistry·2026
Same author

Genetics of growth rate in induced pluripotent stem cells.

Stem cell reports·2026

Related Experiment Video

Updated: Jan 19, 2026

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
08:07

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage

Published on: September 16, 2025

1.1K

Deciphering Metabolic Heterogeneity by Single-Cell Analysis.

Tom M J Evers1, Mazène Hochane2, Sander J Tans3

  • 1Medical Systems Biophysics and Bioengineering, Leiden Academic Centre for Drug Research, Faculty of Mathematics and Natural Sciences , Leiden University , Einsteinweg 55 , 2333 CC Leiden , The Netherlands.

Analytical Chemistry
|September 25, 2019
PubMed
Summary
This summary is machine-generated.

Single-cell analysis reveals cellular differences and dynamics. This work highlights new methods for single-cell metabolic analysis, including mass spectrometry, RNA-seq, and imaging techniques.

More Related Videos

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

7.1K
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
07:41

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

Published on: February 4, 2017

8.9K

Related Experiment Videos

Last Updated: Jan 19, 2026

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
08:07

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage

Published on: September 16, 2025

1.1K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

7.1K
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
07:41

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

Published on: February 4, 2017

8.9K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Analytical Chemistry

Background:

  • Single-cell analysis offers deep insights into cellular heterogeneity and dynamics.
  • Understanding individual cell behavior is crucial for various biological and medical research areas.

Purpose of the Study:

  • To review recent advancements in analytical techniques for single-cell metabolic analysis.
  • To focus on mass spectrometry, RNA-seq, and imaging for studying metabolic stochasticity.

Main Methods:

  • Mass spectrometry-based analytical platforms for metabolic profiling.
  • RNA sequencing (RNA-seq) for gene expression analysis at the single-cell level.
  • Advanced imaging techniques to visualize metabolic processes within individual cells.

Main Results:

  • Highlighting the capabilities of mass spectrometry in detailed single-cell metabolic characterization.
  • Demonstrating the utility of RNA-seq in understanding metabolic gene expression variability.
  • Showcasing imaging methods for revealing spatial and temporal metabolic dynamics.

Conclusions:

  • Emerging analytical techniques are enhancing our ability to study metabolism at the single-cell level.
  • These methods provide critical insights into cellular heterogeneity and metabolic stochasticity.
  • Further development promises to deepen our understanding of cellular functions and disease mechanisms.