Simultaneous measurement of serum 25-hydroxycholecalciferol and 25-hydroxyergocalciferol

A method for the selective measurement of serum 25-hydroxycholecalciferol and 25-hydroxyergocalciferol has been developed. The two hydroxysecosteroids were measured in a control group and in a group of ambulatory patients treated chronically with anticonvulsant drugs. In both groups the serum levels of 25-hydroxycholecalciferol and 25-hydroxyergocalciferol followed a log normal distribution. No significant difference could be elicited between the control and the treated groups for either hydroxysecosteroid. Furthermore the data shows how important is the dietary source of vitamin D in our environment.