Nylon mesh cryodevice for bovine mature oocytes, easily removable excess vitrification solution

Area of Science:

• Veterinary Science
• Reproductive Biology
• Cryobiology

Background:

• Efficient cryopreservation of bovine oocytes is crucial for livestock breeding and genetic resource preservation.
• Current vitrification methods often face challenges with scaling up for large quantities while maintaining high survival rates.

Purpose of the Study:

• To determine the optimal pore size of a nylon mesh (NM) device for the cryosurvival of bovine mature oocytes.
• To evaluate the efficacy of the NM device for vitrifying large quantities of oocytes.

Main Methods:

• Bovine oocytes were loaded onto NM devices with 37-, 57-, or 77-μm pore sizes.
• Oocytes underwent vitrification, warming, in vitro fertilization, and subsequent culture.
• Recovery rates, morphological survival, and blastocyst yields were assessed.

Main Results:

• Oocyte recovery and morphological survival were similar across all pore size groups.
• The 37-μm NM device resulted in a significantly higher blastocyst yield (39%) compared to the 77-μm group (28%).
• The 37-μm NM device successfully vitrified larger quantities (>40 oocytes) with a 33% blastocyst yield.

Conclusions:

• A 37-μm pore size nylon mesh device is suitable for cryosurvival of bovine mature oocytes.
• This NM device facilitates the vitrification of large quantities of in vitro-matured bovine oocytes, improving developmental outcomes.