Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

8.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.3K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

10.4K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.4K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.4K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.4K
Catenins01:23

Catenins

3.0K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
3.0K
Notch Signaling Pathway03:14

Notch Signaling Pathway

6.5K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.5K
Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

7.7K
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
Graded and Abrupt Responses
Some signaling systems generate...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial dysfunction underlies cardiac contractility and growth defects in a zebrafish model of <i>NAA15</i>-related heart disease.

bioRxiv : the preprint server for biology·2026
Same author

Cardiovascular and Cardiometabolic Outcomes in Adults with Fetal Alcohol Spectrum Disorders: A Retrospective Cohort Study.

medRxiv : the preprint server for health sciences·2026
Same author

3D spatial organization of heterogeneous nkx2.5+ progenitors in the zebrafish heart field pre-patterns cardiovascular development.

Nature communications·2025
Same author

Dosage-sensitive <i>RBFOX2</i> autoregulation promotes cardiomyocyte differentiation by maturing the transcriptome.

bioRxiv : the preprint server for biology·2025
Same author

An organ-wide spatiotemporal transcriptomic and cellular atlas of the regenerating zebrafish heart.

Nature communications·2025
Same author

Embryonic alcohol exposure in zebrafish predisposes adults to cardiomyopathy and diastolic dysfunction.

Cardiovascular research·2024

Related Experiment Video

Updated: Jan 19, 2026

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

1.9K

Canonical Wnt Signaling Sets the Pace.

C Geoffrey Burns1, Caroline E Burns2

  • 1Department of Cardiology, Boston Children's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA.

Developmental Cell
|September 25, 2019
PubMed
Summary

Researchers identified the embryonic source of zebrafish cardiac pacemaker cells, finding Wnt5b crucial for their development. This discovery advances the potential for creating biological pacemakers from human stem cells.

More Related Videos

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
07:34

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

8.4K
The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

113.6K

Related Experiment Videos

Last Updated: Jan 19, 2026

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

1.9K
The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
07:34

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

8.4K
The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

113.6K

Area of Science:

  • Developmental biology
  • Cardiovascular research
  • Stem cell science

Background:

  • Cardiac pacemaker cells are essential for heart rhythm.
  • Understanding the precise origin and differentiation of these cells is key to regenerative medicine.

Purpose of the Study:

  • To identify the embryonic origin of cardiac pacemaker cells in zebrafish.
  • To investigate the role of Wnt signaling in pacemaker cell differentiation.
  • To explore the potential for generating functional pacemaker cells from human stem cells.

Main Methods:

  • Zebrafish embryogenesis studies.
  • Gene expression analysis.
  • In vitro differentiation of human cardiac progenitors using Wnt pathway activation.

Main Results:

  • Identified a specific embryonic source for zebrafish cardiac pacemaker cells.
  • Demonstrated that Wnt5b signaling promotes the differentiation of these cells.
  • Showed that canonical Wnt activation can generate functional pacemaker cells from human stem cell-derived cardiac progenitors in vitro.

Conclusions:

  • The embryonic origin and differentiation mechanisms of cardiac pacemaker cells are elucidated.
  • Wnt signaling, particularly Wnt5b, plays a critical role in pacemaker cell development.
  • This research holds significant promise for the future of biological pacemakers in treating cardiac arrhythmias.