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Updated: Jan 19, 2026

Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge
Published on: September 26, 2016
Sludge dewaterability: The variation of extracellular polymeric substances during sludge conditioning with two
Marie Christine Amie Sene Faye1, Kai Kai Zhang1, Sun Peng1
1School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan, Hubei, 430074, PR China; Environmental Science Research Institute, Huazhong University of Science and Technology, Wuhan, 430074, PR China.
Abstract:
To assess the characteristics of Extracellular Polymeric Substances during natural organic conditioners for sludge treatment, Moringa oleifera (MO) and chitosan (CTS) were used as conditioners. The findings of this study show that despite the high EPS content due to the fact that this component is not destroyed upon conditioning, sludge conditioned with MO and CTS displayed efficient and improved dewaterability. MO and CTS showed the same mechanism of action by protonation of the negatively charged EPS and reduction of electrostatic repulsions between sludge flocs, thus enhancing sludge filterability by neutralizing and settling. The effect of MO and CTS on EPS lies in their ability to neutralize EPS, settling them. MO and CTS neutralize and aggregate the EPS, hence improving sludge dewaterability. The results indicate that CTS can impact the EPS quantity and quality while MO impacts the EPS quality. Understanding the impact of MO and CTS on sludge EPS can help elucidate the mechanism of their dewaterability efficiency.
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