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Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
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An efficient and rapid method to extract and purify protein - Liquid Triphasic Flotation system.

Shir Reen Chia1, Kah Yan Mak1, Yee Jian Khaw1

  • 1Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Semenyih 43500, Malaysia.

Bioresource Technology
|September 25, 2019
PubMed
Summary

Microalgae offer a sustainable protein source. Liquid Triphasic Flotation (LTF) efficiently extracts proteins from Chlorella vulgaris, achieving high recovery and separation rates with optimized conditions and reusable solvents.

Keywords:
Chlorella sp.Liquid Triphasic FlotationMicroalgaeProtein extractionRecycling

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Area of Science:

  • Biotechnology
  • Food Science
  • Sustainable Resources

Background:

  • Microalgae are a promising source of valuable biomolecules and alternative proteins.
  • Their rapid growth and ability to utilize non-arable land support sustainable cultivation.
  • Efficient protein extraction is crucial for unlocking the potential of microalgae.

Purpose of the Study:

  • To investigate the Liquid Triphasic Flotation (LTF) system for efficient protein extraction from Chlorella vulgaris.
  • To optimize the conditions for LTF-based protein recovery and separation.
  • To evaluate the performance of LTF compared to the Three Phase Partitioning (TPP) system.

Main Methods:

  • Utilized Liquid Triphasic Flotation (LTF) for protein extraction from Chlorella vulgaris.
  • Optimized parameters including t-butanol concentration, salt solution concentration, biomass loading, pH, and air flotation time.
  • Assessed t-butanol recyclability and compared LTF efficiency with the traditional Three Phase Partitioning (TPP) method.

Main Results:

  • Achieved 87.23% protein recovery and 56.72% separation efficiency under optimized LTF conditions.
  • Identified optimal conditions: 70% t-butanol, 40% salt solution, 0.5% biomass, pH 5.54, 1:1 salt to t-butanol ratio, and 10 min flotation time.
  • Demonstrated that a single run of t-butanol recycling maintained system performance, outperforming the TPP system.

Conclusions:

  • The Liquid Triphasic Flotation (LTF) system is a highly effective method for protein extraction and purification from microalgae.
  • LTF offers advantages over TPP, including fewer operational units and reduced processing time.
  • This technique enhances the viability of microalgae as a sustainable alternative protein source.