Immunobiological relationships between Vibrio fluvialis and Vibrio cholerae enterotoxins

A total of 26 strains of Vibrio fluvialis was included in this study, which were isolated from patients with diarrhoea and other sources. The GM1 enzyme linked immunosorbent assays performed with the culture filtrates of V. fluvialis yielded negative results, indicating that their receptor site is different from that of the known labile toxin. The cholera antitoxin failed to neutralize the skin permeability factor activities of all the V. fluvialis culture filtrates and none of the concentrated culture filtrates gave any precipitin band, when tested against the cholera antitoxin in Ouchterlony's gel diffusion test. These observations suggest that the toxin of V. fluvialis differs from the known cholera toxin in receptor site, mode of action and antigenicity.