Self-Assembled Two-Dimensional Molybdenum Disulfide Nanosheet Geno-Interface for the Detection of Salmonella

This report presents a novel lab-on-a-paper (LoP)-based device coupled with a molybdenum disulfide nanosheet (MoS₂NS)-modified electrochemical genosensor for detecting Salmonella-specific DNA. Conductive electrodes were grafted on a paper-based substrate employing a stencil printing technique, and MoS₂NS was decorated on the working electrode. MoS₂NS has strong affinity toward nucleo bases, which made it a best sensing interface for the immobilization of DNA. Morphological, optical, and structural characterizations were accomplished using X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM), UV-vis spectroscopy (UV-vis), and Raman spectroscopy, repectively. The current studies of an electrochemical genosensor demonstrated a good linear detection range from 100-20 nM and a low limit of detection of 20 nM toward Salmonella DNA with R ² = 0.991. The proposed LoP-based genosensor confirmed as a better sensing podium and an effectual immobilization matrix for DNA.