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Related Experiment Video

Updated: Jan 19, 2026

In Ovo Electroporation in the Chicken Auditory Brainstem

In Ovo Electroporation in the Chicken Auditory Brainstem

Published on: June 9, 2017

Gene Silencing in Chicken Brain Development.

Georgia Tsapara¹, Irwin Andermatt¹, Esther T Stoeckli²

¹Neuroscience Center Zurich, Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)

|September 26, 2019

Summary

Studying gene function in developing brains is challenging. The chicken embryo offers a powerful, accessible model for precise temporal gene silencing, overcoming limitations in mammalian models.

Keywords:

Artificial miRNA Cerebellum Chicken embryo Electroporation Gene silencing Neural development RNA interference

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Last Updated: Jan 19, 2026

In Ovo Electroporation in the Chicken Auditory Brainstem

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Published on: June 9, 2017

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations

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Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

Published on: May 29, 2013

Area of Science:

Developmental biology
Neuroscience
Genetics

Background:

Studying brain development in vivo is limited by current models like iPSC-derived organoids.
Mammalian models face challenges in gene function studies due to developmental timing and potential lethality.
Precise temporal control of gene silencing is crucial for understanding developmental gene function.

Purpose of the Study:

To highlight the advantages of the chicken embryo as a model for studying gene function during embryonic development.
To demonstrate the utility of temporal gene silencing in oviparous models.
To overcome the limitations of mammalian models in developmental gene studies.

Main Methods:

Utilizing the chicken embryo as an accessible oviparous model for developmental studies.
Employing RNA interference (RNAi) and CRISPR/Cas9 gene silencing techniques.
Implementing in ovo and ex ovo culture methods for temporal control of gene silencing.

Main Results:

Chicken embryos provide a powerful platform for experimental manipulation during development.
RNAi and CRISPR/Cas9 enable precise temporal control of gene silencing in chicken embryos.
This approach facilitates the analysis of gene function at specific developmental stages.

Conclusions:

The chicken embryo is a superior model for studying gene function during development compared to mammals.
Temporal gene silencing in chicken embryos allows for detailed analysis of gene roles without early developmental disruption.
This model system enhances our understanding of neurogenesis and developmental processes.