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Updated: Jan 19, 2026

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Published on: August 15, 2011

In Utero Electroporation to Study Mouse Brain Development.

Emilie Pacary^1,2, François Guillemot³

¹INSERM U1215, Neurocentre Magendie, Bordeaux, France. emilie.pacary@inserm.fr.

Methods in Molecular Biology (Clifton, N.J.)

|September 26, 2019

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In utero electroporation is a powerful in vivo method for studying brain development. This technique offers advantages for examining processes from cell proliferation to synaptic integration in various brain regions.

Area of Science:

Neuroscience
Developmental Biology
Molecular Biology

Background:

Studying brain development in vivo is crucial for understanding neurological disorders.
Existing methods have limitations in targeting specific developmental stages or brain regions.

Purpose of the Study:

To provide a detailed protocol for in utero electroporation.
To highlight the versatility of this technique for studying brain development.

Main Methods:

Detailed step-by-step description of the in utero electroporation procedure.
Discussion of variations for targeting different cerebral structures.
Adaptations for studying specific developmental processes (proliferation, migration, differentiation, synaptic integration).

Keywords:

Brain Electroporation Embryo Gain and loss of function In utero Mouse Neuronal development Transfection

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In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos

In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos

Published on: July 19, 2007

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

Published on: June 14, 2020

Related Experiment Videos

Last Updated: Jan 19, 2026

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Published on: August 15, 2011

In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos

In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos

Published on: July 19, 2007

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

Double In Utero Electroporation to Target Temporally and Spatially Separated Cell Populations

Published on: June 14, 2020

Main Results:

Successful application of in utero electroporation across various brain regions.
Demonstration of its utility in dissecting distinct phases of neurodevelopment.
Establishment of a reproducible and adaptable experimental approach.

Conclusions:

In utero electroporation is a rapid, powerful, and advantageous technique for in vivo brain development studies.
The described protocol and its variations enable targeted investigation of neurodevelopmental processes.
This method facilitates research from cellular proliferation to synaptic integration.