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Virus vector-mediated gene transfer.

A J Levine1

  • 1Princeton University, Department of Molecular Biology, Lewis Thomas Laboratory, NJ 08544.

Microbiological Sciences
|August 1, 1987
PubMed
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Animal virus vectors are engineered for efficient foreign gene delivery into host cells. These systems offer safety, specificity, and high production levels for experimental and commercial gene expression applications.

Area of Science:

  • Molecular Biology
  • Virology
  • Gene Therapy

Background:

  • Animal viruses are utilized to create virus vector systems for delivering foreign genes into host cells or organisms.
  • The fundamental requirements for virus vectors, including replication, packaging, and assembly functions, have been identified.
  • Both defective and viable virus vectors can be generated.

Purpose of the Study:

  • To review the development and capabilities of animal virus vector systems for gene delivery.
  • To highlight the advancements in controlling gene expression and localization using these vectors.
  • To emphasize the suitability of virus vectors for experimental and commercial applications.

Main Methods:

  • Utilizing various animal virus groups to construct vector systems.

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  • Defining minimal requirements for vector replication, packaging, and assembly.
  • Incorporating regulatory signals for cell/tissue specificity, transcription, RNA processing, and translation.
  • Engineering vectors for specific localization of foreign gene products within cellular compartments.

    • Main Results:

    • Successful development of diverse virus vector systems from various animal virus groups.
    • Defined minimal functional requirements for effective virus vector systems.
    • Ability to control foreign gene expression with cell/tissue specificity and optimize production.
    • Achieved specific localization of foreign gene products within target cellular compartments.

    Conclusions:

    • Virus vector systems are highly versatile tools for foreign gene delivery.
    • Current advancements enable precise control over gene expression and product localization.
    • The combination of safety, specificity, and high production efficiency makes virus vectors ideal for gene expression applications.