Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jan 19, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

39.8K

Single-Molecule Topochemical Analyses for Large-Scale Multiplexing Tasks.

Shankar Mandal1, Xiaoqing Zhang1,2, Shankar Pandey1

  • 1Department of Chemistry and Biochemistry , Kent State University , Kent , Ohio 44242 , United States.

Analytical Chemistry
|September 26, 2019
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanozyme: An Artificial Enzyme With a Mechanophore Framework.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Kinetically Trapped Ligand Binding in DNA Tandem Repeats.

Biochemistry·2026
Same author

High-Throughput Characterization of Individual Microdroplets Unveils Ultrasound-Enhanced Radical Production.

Journal of the American Chemical Society·2026
Same author

DNA nanodevice for analysis of force-activated protein extension and interactions.

Nature nanotechnology·2025
Same author

Modulating a Massive Set of Biomolecular Structures by Sono-Mechanical Force.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Chiral Matching between Nucleic Acids and Polypeptides Facilitates Liquid-Liquid Phase Separation.

The journal of physical chemistry letters·2025
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
Same journal

Tunable Charge Transfer in Europium Metal-Organic Frameworks for Ratiometric Sensing of a Sarin Simulant.

Analytical chemistry·2026
Same journal

A β-Cyclodextrin/Ag<sub>2</sub>O@MWCNT-Based Stochastic Platform for the Simultaneous Molecular Enantiorecognition and Enantioanalysis of Twelve Amino Acids in Biological Matrices.

Analytical chemistry·2026
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
See all related articles

This study introduces a novel topochemical spatiotemporal analysis for multiplexed biomarker detection. It enables simultaneous analysis of five microRNA biomarkers with high sensitivity and speed.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • Multiplexed analyses are crucial for next-generation diagnostics.
  • Current methods struggle with analyzing more than three tasks due to space and signal-to-noise limitations.

Purpose of the Study:

  • To develop a method for analyzing multiple microRNA biomarkers simultaneously.
  • To overcome the limitations of existing multiplexed analysis techniques.

Main Methods:

  • Spatially encoding microRNA recognition units within a DNA template at nanometer resolution.
  • Utilizing mechanical unfolding of a hairpin structure for temporal decoding of analyte binding.
  • Employing single-molecule techniques for superior signal-to-noise ratio.

More Related Videos

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.6K
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

6.6K

Related Experiment Videos

Last Updated: Jan 19, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

39.8K
Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.6K
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

6.6K

Main Results:

  • Successfully analyzed five microRNA biomarkers simultaneously.
  • Achieved subpicomolar detection limits for microRNAs.
  • Demonstrated temporal decoding of analyte binding within seconds.

Conclusions:

  • The developed topochemical spatiotemporal analysis offers a powerful new approach for massive single-molecule target analysis.
  • This method significantly enhances multiplexing capabilities in bioanalyses.