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Updated: Jan 19, 2026

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
Published on: May 10, 2020
Soil nitrogen dynamics during an oilseed rape (Brassica napus L.) growing cycle in a humid Mediterranean climate
N Villar1,2, M Aranguren2, A Castellón2
1Department of Plant Biology and Ecology, The University of the Basque Country, Barrio Sarriena s/n, 48940, Leioa, Biscay, Spain.
Abstract:
Nitrogen budgets help explain the supply pattern of N from the soil to the crop. Through budgeting, an improvement of the N fertilization strategy can be achieved. The objective of the present study, which was carried out under humid Mediterranean climate conditions, was to assess the influence of N fertilization, temperature and soil humidity on soil N dynamics during a whole oilseed rape growing cycle. A field experiment was conducted with two treatments: without N (0 N) and with application of 180 kg N ha-1(180 N). Mineralization was calculated from N balances made throughout the growing cycle, all while taking into account measured N uptake by oilseed rape and N losses by leaching and N2O emissions. Nitrogen net mineralization was negative after fertilization, reaching -6.73 kg N ha-1, day-1, but total net mineralization over the year was similar for the 0 N and 180 N treatments (21 and 8 kg N ha-1, respectively). Temperatures over 5 °C were sufficient for initiating the mineralization processes. In the summer, when the soil water content was below the wilting point, immobilization took place; however, there is a risk of N leaching if rainfall occurs thereafter, mainly in the 180 N treatment.
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