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Related Experiment Video

Updated: Jan 19, 2026

Microdissection and Whole Mount Scanning Electron Microscopy Visualization of Mouse Choroid Plexus
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Culture Model for Non-human Primate Choroid Plexus.

Elizabeth C Delery1,2,3, Andrew G MacLean1,2,3,4,5

  • 1Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, United States.

Frontiers in Cellular Neuroscience
|September 27, 2019
PubMed
Summary

Researchers developed a new rhesus macaque choroid plexus epithelial cell culture model. This model offers a translationally relevant system for studying central nervous system (CNS) inflammation and blood-cerebrospinal fluid (CSF) barrier function.

Keywords:
agingcell culturechoroid plexusepithelial cellinfectious diseaserhesus macaque

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Immunology

Background:

  • Existing rodent models lack translational relevance for choroid plexus (CP) activation and function.
  • The rhesus macaque is a gold standard for modeling viral CNS infections, including HIV-associated neurocognitive disorders.
  • A need exists for a CP epithelial cell culture model that accurately reflects in vivo conditions for studying inflammation.

Purpose of the Study:

  • To develop and validate a rhesus macaque choroid plexus epithelial cell culture model.
  • To assess the model's suitability for studying central nervous system (CNS) inflammation and blood-cerebrospinal fluid (CSF) barrier function.
  • To investigate the real-time effects of inflammatory mediators on CP epithelial barrier properties.

Main Methods:

  • Established a rhesus macaque choroid plexus epithelial cell culture.
  • Assessed epithelial morphology and function using immunofluorescence (vimentin, phalloidin, ZO-1, FAK) and specific CP markers (transthyretin, α-klotho).
  • Monitored blood-CSF barrier properties in real-time using an xCelligence system with pro- and anti-inflammatory mediators (TNF-α, PamCys3K, dexamethasone).

Main Results:

  • Confirmed choroid plexus epithelial cell identity and integrity using specific markers and proteins.
  • Demonstrated that pro-inflammatory TNF-α decreased barrier function in a dose-dependent manner.
  • Showed that the anti-inflammatory steroid dexamethasone repaired barrier function, and TLR2 agonist PAM3CysK enhanced barrier function in TNF-α treated cells.

Conclusions:

  • A novel rhesus macaque choroid plexus epithelial cell culture model has been successfully developed.
  • This model accurately reflects epithelial cell morphology, function, and barrier properties relevant to CNS inflammation.
  • The model provides a valuable tool for studying inflammatory conditions and real-time changes in the blood-CSF barrier.