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Updated: Jan 19, 2026
Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
Published on: June 26, 2018
In vivo ratiometric tracking of endogenous β-galactosidase activity using an activatable near-infrared fluorescent
Limin Shi1, Chenxu Yan1, Yiyu Ma1
1State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Functional Materials Chemistry, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai 200237, China. guozq@ecust.edu.cn.
Abstract:
Herein, we developed a dual-channel and light-up near-infrared fluorescent probe for ratiometric sensing of β-galactosidase (β-gal) activity. The well-designed probe, which shows ratiometric optical response with a significant red-shift (from 575 nm to 730 nm), was successfully applied to detect endogenous β-gal activity in SKOV-3 cells and tumor-bearing mice.
