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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Updated: Jan 19, 2026

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

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Published on: October 20, 2020

Decoding communication patterns of the innate immune system by quantitative proteomics.

Arjun Sukumaran¹, Jeremia M Coish², Jason Yeung¹

¹Molecular and Cellular Biology Department, University of Guelph, Guelph, Ontario, Canada.

Journal of Leukocyte Biology

|September 27, 2019

Summary

This summary is machine-generated.

Mass spectrometry-based proteomics enables comprehensive profiling of innate immune cells, overcoming challenges like low cell numbers and protein abundance. This technology reveals immune cell communication dynamics for host defense and drug discovery.

Keywords:

activation cellular mapping drug discovery innate immunity leukocytes mass spectrometry-based proteomics phagocytic cells signaling cascades

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

Immunology
Proteomics
Cellular Biology

Background:

The innate immune system relies on complex cell communication for host defense and tissue homeostasis.
Profiling innate immune cells is difficult due to low cell numbers, high protein dynamic range, and protease activity.

Purpose of the Study:

To explore mass spectrometry-based proteomics applications in innate immunity.
To define innate immune cell communication patterns in health and disease.
To provide a technical overview of proteomic workflows and discuss future applications in immune-based drug discovery.

Main Methods:

Mass spectrometry-based proteomics, particularly bottom-up approaches.
Integration of multi-omics datasets and advanced technologies.
Analysis of cellular processes, signaling cascades, post-translational modifications, and interaction networks.

Main Results:

Mass spectrometry-based proteomics overcomes limitations in comprehensive innate immune cell profiling.
Proteomics enables detailed analysis of cellular communication, dynamics, and networks.
Emerging role of proteomics in immune-based drug discovery is highlighted.

Conclusions:

Mass spectrometry-based proteomics is a powerful tool for understanding innate immunity.
Proteomics provides insights into immune cell communication crucial for host defense and homeostasis.
Future applications of proteomics in immune-based drug discovery are promising.