Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Drosophila melanogaster aldehyde dehydrogenase.

M C Liétaert, M Libion-Mannaert, S Wattiaux-De Coninck

    Experientia
    |January 15, 1985
    PubMed
    Summary

    Aldehyde dehydrogenase was detected in Drosophila melanogaster using subcellular fractionation. This enzyme is primarily located within the heavy mitochondrial fraction of the cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    SEXUAL VIGOR AND SEXUAL SELECTION IN DROSOPHILA MELANOGASTER.

    Evolution; international journal of organic evolution·2017
    Same author

    Uptake and intracellular fate of polyethylenimine in vivo.

    Biochemical and biophysical research communications·2000
    Same author

    Endosomes, lysosomes: their implication in gene transfer.

    Advanced drug delivery reviews·2000
    Same author

    Subcellular localization of mannose 6-phosphate glycoproteins in rat brain.

    The Journal of biological chemistry·1999
    Same author

    Uptake by rat liver and intracellular fate of plasmid DNA complexed with poly-L-lysine or poly-D-lysine.

    FEBS letters·1999
    Same author

    Delta F508 CFTR localizes in the endoplasmic reticulum-Golgi intermediate compartment in cystic fibrosis cells.

    Experimental cell research·1998

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Genetics

    Background:

    • Aldehyde dehydrogenase (ALDH) enzymes play critical roles in cellular metabolism and detoxification.
    • Understanding the subcellular localization of ALDH is crucial for elucidating its specific functions.
    • Drosophila melanogaster serves as a model organism for studying fundamental biological processes.

    Purpose of the Study:

    • To confirm the presence and determine the subcellular localization of aldehyde dehydrogenase in Drosophila melanogaster.
    • To investigate the distribution of ALDH within different cellular compartments.

    Main Methods:

    • Subcellular fractionation was employed to isolate various cellular components.
    • Differential centrifugation was utilized to separate fractions based on density and size.
    • Enzyme activity assays or Western blotting were likely used to detect ALDH in fractions.

    Main Results:

    • Subcellular fractionation confirmed the presence of aldehyde dehydrogenase activity in Drosophila melanogaster.
    • The enzyme was predominantly found in the heavy mitochondrial fraction, indicating its primary localization to mitochondria.
    • This suggests a significant role for mitochondrial ALDH in D. melanogaster.

    Conclusions:

    • Aldehyde dehydrogenase is present and primarily localized to mitochondria in Drosophila melanogaster.
    • The mitochondrial localization points to its involvement in metabolic pathways occurring within this organelle.
    • Further research can explore the specific mitochondrial functions of ALDH in this model organism.

    Related Experiment Videos