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Updated: Jan 19, 2026
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
Published on: March 7, 2018
Assembled cationic dipeptide-gold nanoparticle hybrid microspheres for electrochemical biosensors with enhanced
Keqing Wang1, Ziruo Li2, Chenlei Wang1
1Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Abstract:
Molecular assemblies of cationic dipeptide-gold nanoparticle (CDP-AuNP) hybrid microspheres were used to modify cholesterol oxidase electrodes for high-sensitivity cholesterol detection. The cationic dipeptide used here serves as a functional molecule for adsorbing chloroauric acid based on electrostatic interactions and assembly into spherical structures, providing a platform for loading gold nanoparticles (AuNPs), increasing the immobilization load of the enzyme and maintaining the activity of the enzyme as a result of excellent biocompatibility. Moreover, the CDP-AuNP modified cholesterol oxidase electrode has a higher electrocatalytic activity to cholesterol with obvious enhancement in the current response, exhibiting a current response 13 times higher than that of the controlled electrode. The outstanding biocompatibility and increased enzyme load by hybrid microspheres and the good charge-transfer ability of AuNPs in the peptide-based electrode indicate a very attractive perspective in the field of biodevices.
