Related Experiment Video

Updated: Jan 19, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.8K

Complete Genome Sequence of Stenotrophomonas maltophilia Podophage Ponderosa

Alejandra Marquez1, Heather Newkirk1, Russell Moreland1

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|September 28, 2019
PubMed

Insights

We sequenced the Ponderosa phage, which infects Stenotrophomonas maltophilia, a multidrug-resistant bacterium. This genome analysis provides insights into phage-host interactions and potential antimicrobial strategies.

Area of Science:

  • Microbiology
  • Genomics
  • Bacteriology

Background:

  • Stenotrophomonas maltophilia is a Gram-negative bacterium recognized as a multidrug-resistant opportunistic pathogen.
  • The increasing prevalence of multidrug-resistant bacteria necessitates research into novel control mechanisms.

Purpose of the Study:

  • To characterize the genome of the T7-like Stenotrophomonas maltophilia podophage Ponderosa.
  • To provide genomic data that could inform future therapeutic strategies against S. maltophilia infections.

Main Methods:

  • Whole-genome sequencing of the Ponderosa phage.
  • Bioinformatic analysis to identify protein-coding genes and genomic features.

Main Results:

  • The genome of podophage Ponderosa was fully described.
  • The genome contains 54 predicted protein-coding genes.
  • A 493-bp terminal repeat was identified in the Ponderosa genome.

Conclusions:

  • The genomic characterization of podophage Ponderosa offers a foundation for understanding Stenotrophomonas maltophilia-phage interactions.
  • This research contributes to the exploration of phage therapy as a potential alternative to antibiotics for multidrug-resistant infections.

More Related Videos

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR14:07

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

Published on: November 23, 2012

17.0K
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing06:56

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

12.2K

Related Concept Videos

Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
48.3K