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Diet Modulation Restores Autophagic Flux in Damaged Skeletal Muscle Cells.

F M Giordano1, S Burattini, F Buontempo

  • 1Sara Salucci, Department of Biomolecular Sciences, University of Urbino Carlo Bo, Urbino, Italy,

The Journal of Nutrition, Health & Aging
|September 28, 2019
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Summary

Dietary strategies like glutamine supplementation can prevent chemotherapy-induced skeletal muscle damage by preserving autophagy and cell function. This approach helps maintain muscle homeostasis against etoposide effects.

Keywords:
ER stressSkeletal muscle cellschemotherapy treatmentlysosomal activitymitochondria dysfunctionality

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Area of Science:

  • Cell Biology
  • Physiology
  • Biochemistry

Background:

  • Autophagy is a vital cellular process for maintaining homeostasis.
  • Impaired autophagy is linked to muscle diseases like muscular dystrophies and sarcopenia.
  • Chemotherapy can induce skeletal muscle damage by disrupting autophagy.

Purpose of the Study:

  • To investigate dietary strategies for preventing chemotherapy-induced skeletal muscle cell damage.
  • To evaluate the efficacy of glutamine supplementation and nutrient deprivation against etoposide toxicity.

Main Methods:

  • In vitro skeletal muscle cell model exposed to etoposide.
  • Pre-treatments included glutamine supplementation and nutrient deprivation.
  • Evaluations involved morpho-functional, cytofluorimetric, and molecular analyses.

Main Results:

  • Etoposide treatment caused mitochondrial dysfunction, ER stress, and lysosomal damage in skeletal muscle cells.
  • Both glutamine supplementation and nutrient deprivation protected myofibers from etoposide-induced damage.
  • Glutamine supplementation preserved cell ultrastructure and lysosomal activity.

Conclusions:

  • Dietary modulation, particularly glutamine supplementation, is a viable strategy to counteract chemotherapy-induced muscle damage.
  • Glutamine preserves skeletal muscle homeostasis by preventing autophagic impairment.
  • This research highlights a potential nutritional intervention for managing chemotherapy side effects on muscle.