Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrathin polymer membranes with locked intrinsic microporosity for hydrocarbon fractionation.

Science (New York, N.Y.)·2026
Same author

Exposure to source-specific VOCs around the Houston Ship Channel.

Environmental research·2026
Same author

Mitigation of acrylamide in cookies through partial replacement of conventional sugars with erythritol and maltitol.

Food chemistry·2026
Same author

In Situ Ptychographic X-ray Computed Tomography of Fully Hydrated Polyamide Membranes.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Gallium-doped zinc oxide semiconductor nanoparticles for plasmonic applications: a combined experimental and computational study.

Nanoscale advances·2026
Same author

Effects of surface-exposed sialic acid and LOS outer core on the ability of native Neisseria meningitidis outer membrane vesicles (nOMVs) to induce cytokine expression and pyroptotic pathways in THP-1-derived macrophages.

Scientific reports·2025

Related Experiment Video

Updated: Jan 18, 2026

Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders
10:10

Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders

Published on: December 4, 2020

2.2K

3D Analysis of Ordered Porous Polymeric Particles using Complementary Electron Microscopy Methods.

Juan Alvarez1, Giovanni Saudino1,2, Valentina Musteata1

  • 1King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division (BESE), Advanced Membranes and Porous Materials Center, 23955-6900, Thuwal, Saudi Arabia.

Scientific Reports
|September 29, 2019
PubMed
Summary
This summary is machine-generated.

Advanced electron microscopy techniques visualize complex porous particle structures for protein sorption. Serial block face scanning electron microscopy proved highly effective for imaging nanoporous block copolymer particles.

More Related Videos

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

11.5K
Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
07:41

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

8.0K

Related Experiment Videos

Last Updated: Jan 18, 2026

Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders
10:10

Three-Dimensional Particle Shape Analysis Using X-ray Computed Tomography: Experimental Procedure and Analysis Algorithms for Metal Powders

Published on: December 4, 2020

2.2K
Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

11.5K
Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
07:41

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

8.0K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomaterials

Background:

  • Highly porous particles with internal triply periodic minimal surfaces are promising for protein sorption.
  • Visualizing their complex internal morphology requires advanced 3D imaging techniques beyond 2D projections.

Purpose of the Study:

  • To investigate the application of advanced electron microscopy methods for 3D imaging of porous particles.
  • To compare the capabilities of transmission electron microscopy (TEM) tomography, focused ion beam (FIB)-SEM, and serial block face (SBF)-SEM for reconstructing complex morphologies.

Main Methods:

  • Transmission electron microscopy (TEM) tomography
  • Focused ion beam (FIB) scanning electron microscopy (SEM)
  • Serial block face (SBF) scanning electron microscopy (SEM)

Main Results:

  • TEM tomography offers high resolution (<1 nm) but limited volume (2.5 μm³).
  • FIB-SEM and SBF-SEM allow in situ sectioning and larger imaging volumes.
  • SBF-SEM was particularly effective for visualizing block copolymer particles (32-54 nm nanopores) with a 20 μm³ sampling volume, demonstrating its utility for synthetic polymers.

Conclusions:

  • Advanced 3D electron microscopy techniques are essential for characterizing complex porous materials.
  • SBF-SEM is a powerful tool for imaging nanoporous synthetic polymers, previously underutilized in this field.
  • These methods enable detailed structural analysis crucial for applications like protein sorption.