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Nestin Null Mice Show Improved Reversal Place Learning.

Ulrika Wilhelmsson¹, Marie Kalm², Marcela Pekna^3,4,5

¹Laboratory of Astrocyte Biology and CNS Regeneration, Center for Brain Repair, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Box 440, 40530, Gothenburg, Sweden.

Neurochemical Research

|September 29, 2019

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Nestin-deficient mice show enhanced reversal learning and memory extinction in a novel home cage task. This suggests nestin plays a complex role in hippocampal neurogenesis and cognitive function.

Area of Science:

Neuroscience
Cell Biology

Background:

Nestin is an intermediate filament protein expressed in neural stem cells and some astrocytes within the adult rodent hippocampus.
Previous studies indicated nestin-deficient (Nes-/-) mice exhibit increased adult hippocampal neurogenesis, reduced Notch signaling, and impaired long-term memory.

Purpose of the Study:

To investigate the impact of nestin deficiency on learning and memory using the IntelliCage system.
To further elucidate the role of nestin in hippocampal function and cognitive processes.

Main Methods:

Assessment of place learning and reversal learning in Nes-/- and wildtype mice within a home cage IntelliCage setup.
Analysis of learning parameters including incorrect corner visit ratio, incorrect nose poke ratio, and memory extinction.

Keywords:

Astrocytes Hippocampal neurogenesis Hippocampus Intermediate filaments Nestin Neural stem cells

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Main Results:

Nes-/- mice demonstrated comparable initial place learning to wildtype mice.
During reversal learning, Nes-/- mice showed improved learning over time and enhanced memory extinction.
Increased exploratory activity, measured by corner visits and nose pokes, was observed in Nes-/- mice.

Conclusions:

Nestin deficiency in mice leads to improved reversal place learning and memory extinction.
These findings support a dual role for hippocampal neurogenesis in cognitive functions, influenced by nestin expression.