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Related Experiment Videos

Benzodiazepines both enhance gamma-aminobutyrate responses and decrease calcium action potentials in guinea-pig

E Cherubini, R A North

    Neuroscience
    |January 1, 1985
    PubMed
    Summary

    Benzodiazepines like midazolam and diazepam enhance gamma-aminobutyrate responses in guinea-pig myenteric neurons at low concentrations. Higher doses and specific conditions reveal a secondary effect of depressing action potentials, independent of gamma-aminobutyrate.

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    Area of Science:

    • Neuroscience
    • Pharmacology
    • Gastroenterology

    Background:

    • The myenteric plexus controls gastrointestinal motility.
    • Benzodiazepines are known for their effects on the central nervous system.
    • Understanding their peripheral actions is crucial.

    Purpose of the Study:

    • To investigate the effects of midazolam and diazepam on guinea-pig myenteric neurons.
    • To characterize the mechanisms underlying these effects.
    • To explore the interaction with gamma-aminobutyrate and specific antagonists.

    Main Methods:

    • Intracellular recording techniques in guinea-pig myenteric neurons.
    • Application of midazolam and diazepam at varying concentrations.
    • Ionophoresis of gamma-aminobutyrate.

    Related Experiment Videos

  • Use of Ro 15-1788 and pentylenetetrazol as antagonists.

    • Main Results:

    • Low concentrations (100-300 pM) of midazolam and diazepam potentiated gamma-aminobutyrate-induced depolarization.
    • Higher concentrations ( >100 nM) depressed this response.
    • A subset of neurons showed a reversible depression of action potential amplitude and duration by benzodiazepines.
    • This action potential effect was independent of gamma-aminobutyrate and partially sensitive to antagonists.

    Conclusions:

    • Benzodiazepines exert dual effects on myenteric neurons: enhancing GABAergic transmission and depressing calcium action potentials.
    • The action potential effect appears distinct from GABAergic modulation.
    • These findings highlight potential peripheral roles for benzodiazepines.