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Updated: Jan 18, 2026

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
Published on: June 13, 2021
PDR-like ABC systems in pathogenic fungi
Alexis Moreno1, Atanu Banerjee2, Rajendra Prasad2
1Drug Resistance & Membrane Proteins Group, Molecular Microbiology and Structural Biochemistry Laboratory, CNRS-Lyon 1 University Research Lab n° 5086, Institut de Biologie et Chimie des Protéines, Lyon, France.
Abstract:
ABC transporters of the Pleiotropic Drug Resistance (PDR) family are the main actors of antifungal resistance in pathogenic fungi. While their involvement in clinical resistant strains has been proven, their transport mechanism remains unclear. Notably, one hallmark of PDR transporters is their asymmetry, with one canonical nucleotide-binding site capable of ATP hydrolysis while the other site is not. Recent publications reviewed here show that the so-called "deviant" site is of crucial importance for drug transport and is a step towards alleviating the mystery around the existence of non-catalytic binding sites.
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