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Updated: Jan 18, 2026
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Ultraselective antibiotic sensing with complementary strand DNA assisted aptamer/MoS2 field-effect transistors
Xiaoyan Chen1, Sibei Hao1, Boyang Zong1
1Biomedical Multidisciplinary Innovation Research Institute, Shanghai East Hospital, State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
This study introduces a novel sensor for detecting kanamycin, an antibiotic. The device uses a molybdenum disulfide (MoS2) field-effect transistor with engineered DNA probes for highly selective and sensitive antibiotic determination.
Area of Science:
- Materials Science
- Biotechnology
- Analytical Chemistry
Background:
- Aptamers are effective probes for antibiotic determination, but selectivity remains a challenge due to structural similarities and aptamer folding.
- Existing methods struggle with precise differentiation between various antibiotics.
Purpose of the Study:
- To develop a highly selective and reliable sensor for kanamycin determination.
- To investigate a novel sensing mechanism for label-free antibiotic detection using field-effect transistors.
Main Methods:
- Fabrication of a field-effect transistor (FET) sensor utilizing a MoS2 nanosheet channel.
- Engineering of an aptamer DNA (APT) probe configured by a complementary strand DNA (CS) for enhanced selectivity.
- Investigation of time-dependent sensing performance and a proposed charge release mechanism based on a replacement reaction.
Main Results:
- The MoS2/APT/CS sensor demonstrated enhanced selectivity and reliability for kanamycin detection.
- Optimized detection times influenced sensitivity and linear working range, with prolonged times yielding higher sensitivity.
- The sensor exhibited exceptional selectivity for kanamycin over other tested antibiotics, with a selectivity coefficient of 12.8.
Conclusions:
- Probe engineering in FET sensors significantly improves label-free antibiotic detection.
- The developed sensor shows great promise for sensitive and selective chemical and biological sensing applications.
- The proposed charge release mechanism offers new insights into aptasensor operation.
