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Highly divergent cattle hepacivirus N in Southern Brazil
M S Da Silva1, M N Weber1, L F Baumbach1
1Laboratório de Virologia, Faculdade de Veterinária (FAVET), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
Archives of Virology
|September 30, 2019
Summary
A novel bovine hepacivirus (HNV) strain was identified in Brazil, exhibiting significant genetic divergence from known HNV sequences. This discovery highlights the extensive genetic variability within hepaciviruses.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- The genus Hepacivirus encompasses 14 species, including Hepacivirus N (HNV) found in cattle.
- Previous HNV isolates displayed high nucleotide sequence identity (80-99.7%) in the NS3 coding region.
Purpose of the Study:
- To characterize a highly divergent bovine hepacivirus isolate from Southern Brazil.
- To investigate the genetic variability and evolutionary landscape of hepaciviruses.
Main Methods:
- Partial genome sequencing of a bovine hepacivirus isolate.
- Phylogenetic analysis of the NS3 coding region.
- Nucleotide sequence identity comparison with known HNV strains.
Main Results:
- A bovine hepacivirus isolate with 72.6% to 73.8% nucleotide sequence identity to known HNV NS3 sequences was identified.
- Phylogenetic analysis indicated this isolate represents a distinct HNV genotype.
- The study identified a significant divergence in the NS3 coding region of the novel HNV isolate.
Conclusions:
- The findings expand the understanding of hepacivirus genetic diversity.
- This divergent HNV isolate suggests a greater evolutionary capacity within the hepacivirus genus.
- Further research is warranted to explore the prevalence and characteristics of this new HNV genotype.
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