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Genetic typing of South Kazakhstan populations' dairy camels using DNA technology
Adilbekova Elmira1, Alybayev Nuradin1, Arunas Svitojus2
1Chair of Biotechnology, M. Auezov South Kazakhstan State University, Shymkent, Republic of Kazakhstan.
Abstract:
The article presents results of genetic typing of South Kazakhstan populations' dairy camels based on DNA technology using 12 camel microsatellite loci (LCA-8, LCA-19, LCA-37, LCA-56, LCA-65, LCA-66, CMS-16, YWLL-08, YWLL-29, YWLL-38, YWLL-44 and VOLP-10). The work shows the effectiveness of the used microsatellite loci in compiling the genetic profile of Kazakh Bactrian and Arvana camel populations bred in Karatau-Moiynkum and Arys-Turkestan zones of South Kazakhstan. The allele pool of the following three populations was studied using microsatellite loci: Arvana breed ('Ussenov N.' farm and 'Syzdykbekov A.' LLP) and Kazakh Bactrian breed ('Bagdat' farm). The level of genetic diversity was determined and the degree of heterozygosity of different camel populations was shown. The characteristics of the studied camel populations are given in terms of F-statistics: according to the degree of inbreeding and the level of interpopulation and intrapopulation diversity in each of the studied camel populations. For the first time in Kazakhstan, the genotyping of South Kazakhstan populations' dairy camels was carried out using DNA technology, which provides a reliable assessment of the genetic profile of highly productive individuals by microsatellite loci for development of dairy industry in different regions of the camel breeding location.
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