Chiral MoS₂ Quantum Dots: Dual-Mode Detection Approaches for Avian Influenza Viruses

Molybdenum disulfide (MoS₂), a type of transition metal dichalcogenide material, has emerged as an important class among 2D systems. When 2D MoS₂ materials are reduced to 0D quantum dots (QDs), they introduce new optical properties that point to several potential technological advantages in electronic, magnetic, optical, and catalytic properties. In this study, a simple way to produce chiral MoS₂ QDs from MoS₂ nanopowder is presented using l(+)-ascorbic acid as a reducing agent. The calculated quantum yield of QDs is 11.06%. Experimental results reveal that the size of QDs is uniformly monodispersed (2-3 nm) and have a blue emissive fluorescence peak and circular dichroism (CD) peak located at 420 and 330 nm, respectively. Furthermore, a dual-mode detection system based on fluorescence and chirality is performed using as-synthesized MoS₂ QDs, where QDs are conjugated with anti-hemagglutinin antibodies of avian influenza virus and made into an immunobridge in the presence of target virus and anti-neuraminidase antibodies conjugated magnetic nanoparticles (MNPs). The photoluminescence and CD spectra of unconjugated QDs after separated magnetochirofluorescent (MNPs-QDs) nanohybrids by external magnets enables influenza virus A (H5N1) detection with the limit of detection value of 7.35 and 80.92 pg mL^-1, respectively.