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Updated: Jan 18, 2026
Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022
A Comparison of Isolation Methods for Black Fungi Degrading Aromatic Toxins
Yu Quan1,2,3, Bert Gerrits van den Ende4, Dongmei Shi5
1Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou and Guizhou Talent Base for Microbiology and Human Health, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China.
Abstract:
The prevalence of black fungi in the order Chaetothyriales has often been underestimated due to the difficulty of their isolation. In this study, three methods which are often used to isolate black fungi are compared. Enrichment on aromatic hydrocarbon appears effective in inhibiting growth of cosmopolitan microbial species and allows appearance of black fungi. We miniaturized the method for high-throughput purposes. The new procedure saves time, consumes less space and can process multiple samples simultaneously.
