Relationship between membrane-bound protein kinase C activity and calcium-dependent proliferation of BALB/c 3T3 cells

Extracellular calcium-deprivation inhibited the proliferation of BALB/c 3T3 cells and this inhibition correlated with a loss of protein kinase C activity from the particulate fraction. Addition of calcium induced proliferation of the cells with the DNA synthetic activity returning to the control rate at 18 hours following calcium addition. The level of protein kinase C activity in the particulate fraction was monitored at various times after calcium addition and increased in parallel with the DNA synthetic activity.