Cardiovascular effects of diesel exhaust inhalation: photochemically altered versus freshly emitted in mice

This study was designed to compare the cardiovascular effects of inhaled photochemically altered diesel exhaust (aged DE) to freshly emitted DE (fresh DE) in female C57Bl/6 mice. Mice were exposed to either fresh DE, aged DE, or filtered air (FA) for 4 hr using an environmental irradiation chamber. Cardiac responses were assessed 8 hr after exposure utilizing Langendorff preparation with a protocol consisting of 20 min of perfusion and 20 min of ischemia followed by 2 hr of reperfusion. Cardiac function was measured by indices of left-ventricular-developed pressure (LVDP) and contractility (dP/dt) prior to ischemia. Recovery of post-ischemic LVDP was examined on reperfusion following ischemia. Fresh DE contained 460 µg/m³ of particulate matter (PM), 0.29 ppm of nitrogen dioxide (NO₂) and no ozone (O₃), while aged DE consisted of 330 µg/m³ of PM, 0.23 ppm O₃ and no NO₂. Fresh DE significantly decreased LVDP, dP/dt_max, and dP/dt_min compared to FA. Aged DE also significantly reduced LVDP and dP/dt_max. Data demonstrated that acute inhalation to either fresh or aged DE lowered LVDP and dP/dt, with a greater fall noted with fresh DE, suggesting that the composition of DE may play a key role in DE-induced adverse cardiovascular effects in female C57Bl/6 mice.