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Microbiota of Children With Complex Appendicitis: Different Composition and Diversity of The Microbiota in Children

Sarah-May M L The1, Roel Bakx1, Andries E Budding2

  • 1From the Department of Paediatric Surgery, Emma Children's Hospital.

The Pediatric Infectious Disease Journal
|October 1, 2019
PubMed

Insights

The appendix microbiome differs between simple and complex appendicitis in children. Complex appendicitis shows increased bacterial intensity and diversity, suggesting a role in disease pathogenesis.

Area of Science:

  • Microbiology
  • Pediatric Surgery
  • Gastroenterology

Background:

  • Appendicitis presents as simple or complex types, necessitating distinct treatment strategies.
  • Understanding the underlying pathogenesis is crucial for differentiating appendicitis types.

Purpose of the Study:

  • To investigate differences in appendix microbial composition between children with simple and complex appendicitis.

Main Methods:

  • A prospective cohort study involved 40 children undergoing appendectomy for suspected appendicitis.
  • Appendix tissue underwent IS-pro analysis to identify bacterial species.
  • Cluster analysis and statistical tests compared microbial profiles in simple versus complex appendicitis.

Main Results:

  • Appendicitis was confirmed in 36 patients; cluster analysis revealed distinct microbial profiles.
  • Complex appendicitis showed significantly increased bacterial intensity and diversity across several phyla.
  • Specific bacterial species, including Bacteroides fragilis and Escherichia coli, were more abundant in complex appendicitis.

Conclusions:

  • Significant differences in microbial composition and diversity exist between simple and complex appendicitis in children.
  • These findings highlight the role of specific bacteria in appendicitis pathogenesis.
  • Further research is needed to explore diagnostic and therapeutic implications.
Abstract

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