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Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA
Published on: February 9, 2011
Panton-Valentine Positive Staphylococcus aureus in Community-Acquired and Hospital-Acquired Pediatric Infections
Maria Fernanda Bádue Pereira1,2, Silvia Yumi Bando3, Suzethe Matiko Sasagawa4
1From the Department of Pediatrics, Division of Pediatric Infectious Disease Santa Casa School of Medicine, São Paulo, Brazil.
Abstract:
From July 2009 to July 2015, Staphylococcus aureus isolated from pediatric sterile sites were selected. Polymerase chain reaction was used to detect mecA and lukS-PV/lukF-PV genes. The rate of methicillin-resistant Staphylococcus aureus was 37.7%. Ten isolates had the lukS-PV/lukF-PV genes, 2 of which were methicillin-resistant Staphylococcus aureus. Skin and soft tissues infections were significantly associated with lukS-PV/lukF-PV positive isolates, P = 0.008.
Insights
Methicillin-resistant Staphylococcus aureus (MRSA) infections in children are a concern. This study found that the lukS-PV/lukF-PV genes were associated with skin and soft tissue infections in pediatric MRSA cases.
Area of Science:
- Clinical Microbiology
- Infectious Diseases
- Pediatric Infectious Diseases
Background:
- Staphylococcus aureus is a common cause of pediatric infections.
- Methicillin-resistance in Staphylococcus aureus (MRSA) presents a significant treatment challenge.
- The presence of specific virulence factors, like lukS-PV/lukF-PV genes, may influence MRSA pathogenicity and clinical presentation.
Purpose of the Study:
- To investigate the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in pediatric sterile site infections.
- To determine the presence and significance of lukS-PV/lukF-PV genes in pediatric Staphylococcus aureus isolates.
- To explore the association between lukS-PV/lukF-PV gene carriage and specific infection types in children.
Main Methods:
- Retrospective analysis of Staphylococcus aureus isolates from pediatric sterile sites between July 2009 and July 2015.
- Polymerase chain reaction (PCR) was employed to detect the mecA gene (conferring methicillin resistance) and the lukS-PV/lukF-PV genes (encoding Panton-Valentine leukocidin).
- Statistical analysis was performed to assess associations between gene presence and clinical outcomes.
Main Results:
- The overall rate of methicillin-resistant Staphylococcus aureus (MRSA) among pediatric isolates was 37.7%.
- Ten isolates harbored the lukS-PV/lukF-PV genes, with two of these also being MRSA.
- A statistically significant association (P = 0.008) was observed between the presence of lukS-PV/lukF-PV genes and skin and soft tissue infections.
Conclusions:
- Methicillin-resistant Staphylococcus aureus (MRSA) is prevalent in pediatric sterile site infections.
- The lukS-PV/lukF-PV genes are present in a subset of pediatric Staphylococcus aureus isolates.
- Carriage of lukS-PV/lukF-PV genes is significantly linked to skin and soft tissue infections in the pediatric population, suggesting a role in localized disease presentation.
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