Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mutations in bacteriophage lambda repressor that prevent RecA-mediated cleavage.

F S Gimble, R T Sauer

    Journal of Bacteriology
    |April 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Structure and dynamics of the tetrameric mnt repressor and a model for its DNA complex.

    Journal of biomolecular structure & dynamics·2012
    Same author

    NMR structure determination of the tetramerization domain of the Mnt repressor: An asymmetric alpha-helical assembly in slow exchange.

    Journal of biomolecular NMR·2010
    Same author

    Protein-Protein Interactions in DNA Recognition: H-NMR Studies of Lambda cI Repressors Genetically Altered by Site-Directed Mutagenesis.

    Biophysical journal·2009
    Same author

    Assignments of the 1H,13C, and 15N resonances of the substrate-binding SSD domain from Lon protease.

    Journal of biomolecular NMR·2002
    Same author

    Simultaneous and functional binding of SmpB and EF-Tu-TP to the alanyl acceptor arm of tmRNA.

    Journal of molecular biology·2001
    Same author

    Degeneration of a homing endonuclease and its target sequence in a wild yeast strain.

    Nucleic acids research·2001
    Same journal

    The bacterial SOS response promotes the expression of the transposase encoded by IS<i>CR</i> mobile genetic elements.

    Journal of bacteriology·2026
    Same journal

    Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

    Journal of bacteriology·2026
    Same journal

    Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

    Journal of bacteriology·2026
    Same journal

    DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

    Journal of bacteriology·2026
    Same journal

    Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

    Journal of bacteriology·2026
    Same journal

    Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

    Journal of bacteriology·2026
    See all related articles

    Researchers identified 16 mutations in the lambda repressor gene that prevent phage induction. These mutations significantly reduce spontaneous and UV-induced prophage activation, impacting bacterial immunity.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Virology

    Background:

    • Bacteriophage lambda repressor controls lysogenic or lytic cycles.
    • Prophage induction, often triggered by DNA damage, leads to viral replication.
    • Understanding repressor function is key to controlling phage-host interactions.

    Purpose of the Study:

    • To isolate and characterize mutations affecting lambda repressor's induction phenotype.
    • To investigate the role of specific repressor mutations in prophage stability.
    • To analyze the impact of these mutations on bacterial immunity.

    Main Methods:

    • Isolation of lambda repressor mutants.
    • Sequence analysis of identified mutations.
    • Characterization of mutant lysogens for spontaneous and UV-induced induction frequencies.

    Related Experiment Videos

  • Assessment of operator-binding properties of mutant repressors.

    • Main Results:

    • 16 distinct mutations were identified at 13 different sites in the repressor gene.
    • Mutant lysogens exhibited significantly reduced spontaneous induction.
    • Mutant lysogens showed minimal to no prophage induction after UV irradiation.
    • Most mutants retained wild-type operator-binding affinity, suggesting induction defects are not due to impaired DNA binding.

    Conclusions:

    • Specific mutations in the lambda repressor gene can confer an induction-deficient phenotype.
    • These mutations stabilize the lysogenic state by preventing prophage activation.
    • The findings provide insights into the regulatory mechanisms of phage-host interactions and bacterial immunity.