Cation and anion effect on the biodegradability and toxicity of imidazolium- and choline-based ionic liquids

This work studies the effect of the cation and anion on the biodegradability and inhibition of imidazolium- and choline-based ionic liquids (ILs) using activated sludge. Six commercial ILs, formed by combination of 1-Butyl-3-methylimidazolium (Bmim⁺) and N,N,N-trimethylethanolammonium (Choline⁺) cations and chloride (Cl^-), acetate (Ac^-) and bis(trifluoromethanesulfonyl)imide (NTf₂^-) anions were evaluated, all representative counter-ions with markedly different toxicity and biodegradability. Inherent and fast biodegradability tests were used to evaluate both the microorganism inhibition and the IL biodegradability. In addition, the ecotoxicological response (EC₅₀) of the ILs was studied using activated sludge and Vibrio fischeri (Microtox® test). Bmim⁺ and NTf₂^- can be considered as non-biodegradable, whereas aerobic microorganisms easily degraded Choline⁺ and Ac^-. The biodegradation pattern of each cation/anion is nearly unaffected by counter-ion nature. Moreover, concentrations of CholineNTf₂ higher than 50 mg/L caused a partial inhibition on microbial activity, in good concordance with its low EC₅₀ (54 mg/L) measured by respiration inhibition test, which alerts on the negative environmental impact of NTf₂-containing ILs on the performance of sewage treatment plants.