Related Experiment Video

Updated: Aug 3, 2026

Detection of True IgE-expressing Mouse B Lineage Cells
09:40

Detection of True IgE-expressing Mouse B Lineage Cells

Published on: December 1, 2014

Subclass specificity of Fc gamma receptor-mediated inhibition of mouse B cell activation

N E Phillips, D C Parker

    Journal of Immunology (Baltimore, Md. : 1950)
    |May 1, 1985
    PubMed

    We have shown previously that cross-linking receptors for the Fc of IgG (Fc gamma R) to membrane Ig will inhibit membrane Ig-dependent mouse B cell activation. In this report we have determined which mouse IgG subclasses will mediate this inhibition by using monoclonal mouse anti-p-azophenylarsonate (anti-ars) antibodies to inhibit B cell activation by arsanilated rabbit Fab'2 anti-mouse IgM (arsFab'2 anti-mu). Cell recovery and Ig secretion after 4 days of culture with soluble helper factors and arsFab'2 anti-mu was inhibited by IgG1, IgG2b, and IgG2a anti-ars, but not IgM or Fab'2 anti-ars. In addition, we determined which of the inhibitory IgG subclasses are blocked by the monoclonal anti-mouse Fc gamma R antibody 2.4G2, which we have previously shown prevents inhibition by rabbit IgG anti-mu. These experiments demonstrated that 2.4G2 Fab could block inhibition mediated by IgG2a as well as that mediated by IgG1 and IgG2b. Because it has been reported that 2.4G2 does not bind the IgG2a receptor on macrophages, these data support our previous experiments, which indicated that this inhibition is not macrophage dependent.

    More Related Videos

    Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries08:49

    Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

    Published on: January 22, 2019

    Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection09:11

    Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

    Published on: July 16, 2019

    Related Concept Videos

    Receptor Downregulation in MVBs01:15

    Receptor Downregulation in MVBs

    Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
    The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...
    T Cell Activation and Clonal Selection01:22

    T Cell Activation and Clonal Selection

    T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
    Naive T cells that have not yet encountered an antigen express two primary CD...
    B Cell Activation and Differentiation01:24

    B Cell Activation and Differentiation

    The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
    When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...